Modification of the TRX2 gene dose in Saccharomyces cerevisiae affects the transcriptome and proteome during wine yeast biomas

21 In the industrial yeast biomass production process, cells undergo an oxidative and other 22 stresses which worsens the quality of the produced biomass. The overexpression of the 23 thioredoxin codifying gene TRX2 in a wine Saccharomyces cerevisiae strain increases 24 resistance to oxidative stress and industrial biomass production yield. We observed that 25 variations in the TRX2 gene dose in wine yeast strains are relevant to determine the 26 fermentative capacity throughout the industrial biomass production process. So we 27 studied the molecular changes using a transcriptomic approach under these conditions. 28 The results provide an overview of the different metabolic pathways affected during 29 industrial biomass production by TRX2 gene manipulation. The oxidative stress-related 30 genes, like those related with the glutathione metabolism, presented outstanding 31 variations. The data also allowed us to propose new thioredoxin targets in S. cerevisiae, 32 such as Hexokinase 2, with relevance for industrial fermentation performance. 33